Effect of intestinal autochthonous Enterococcus faecalis on the growth performance, gut morphology of Malaysian mahseer (Tor tambroides) and protection against Aeromonas hydrophila

Document Type : Original Article

Authors

1 Higher Institution Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries (AKUATROP), Malaysia

2 Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

3 Alternative Aquaculture, Melbourne, Australia

Abstract

The  study  aimed  to  investigate  the  effects  of  dietary  supplementation  of  two  strains  of  potential intestinal  autochthonous  probiotics,  Enterococcus faecalis  on  growth  performances,  body  composition, gut morphology of Malaysian mahseer,  Tor tambroides,  and  survival  of  the  juveniles  challenged  with Aeromonas  hydrophila.  In  this  study,  270  fish  were  fed  for  70  days  with  three  different  diets;  a  control (CON)  and  two  diets  supplemented  with  E. faecalis  (1x10 8  CFUg-1)  as strains 2674 (T1) and FC11682 (T2).  At  the  end  of  the  study,  growth  performance,  whole  body  fatty  acid  profiles,  and  gut  morphology (villus  length,  width,  and  villus  area)  were  significantly  (P  <0.05)  increased  in  probiotic  treatment  groups. The  highest  (P  <0.05)  weight  gain  (%),  SGR  (%  day -1),  and  FCR  were  observed  in  T2.  Additionally,  the mortality  after  challenge  test  was  100%  in  the  control  group  (CON)  whereas  values  were  the  lowest  (30%) (P  <0.05)  in  T2  followed  by  T1  (40%).  Both  the  T1  and  T2  showed  significantly  improved  growth,  gut morphology, and protection against  A.  hydrophila,  with  E. faecalis  strain FC11682 (T2) being the most recommended of tested probiotics for Malaysian mahseer.

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