Characterization of antioxidant and surface active properties of gelatin hydrolysates obtained from croaker fish skin

Document Type: Original Article


1 ICAR-Central Institute of Fisheries Technology, Cochin 652029, India

2 Fish Processing Division, ICAR-Central Institute of Fisheries Technology, Cochin 682029, India

3 Department of Fish Processing Technology, Karnataka Veterinary, Animal and Fisheries Sciences University, Bidar, College of Fisheries, Mangalore 575002, India


In this study, antioxidant and surface-active properties of gelatin hydrolysates (GH) were evaluated. Gelatin obtained from croaker fish (Johnius dussumieri) skin was hydrolysed by the application of visceral proteases extracted from gastrointestinal (GI) tract of two freshwater fish species rohu (Labeo rohita)and catla (Catla catla). The resultant GH was designated as ‘gelatin hydrolysate-rohu protease’ (GH-RP) and ‘gelatin hydrolysate-catla protease’ (GH-CP). Surface-active properties (foaming and emulsion) of GH-RP were significantly higher than GH-CP at concentrations studied (P < 0.05). The GH obtained by enzymatic hydrolysis treatment was fractionated using Ultrafiltration and a total number of five fractions were collected unfractionated (UNF), >10 kDa, 5-10 kDa, 3-5 kDa, 1-3 kDa and 50 value of DPPH radical scavenging activity of fraction 1-3 kDa from GH-RP and fraction