Optimization of enzymatic hydrolysis of visceral waste proteins of beluga sturgeon Huso huso using Alcalase

Document Type : Original Article


1 Department of Fisheries, Gorgan Agricultural Sciences and Natural Resources University, Iran; Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway

2 2Department of Chemistry, Islamic Azad University of Tonekabon, Tonekabon, Iran

3 Department of Food Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

4 Department of Food Science and Human Nutrition, Washington State University, Washington,USA

5 Shahid Rajaee Sturgeon Fish Farm, Sari, Iran


Fish protein hydrolysate (FPH) was produced from the viscera of beluga sturgeon (Huso huso). Using response surface methodology (RSM) and a factorial design to minimize enzyme use and to model the degree of hydrolysis (r2 = 0.94), the hydrolysis conditions (temperature, time and enzyme activity) were optimized. The optimum conditions were: 50°C, 120 min, protease (Alcalase® 2.4 L) activity on 34 AU/kg protein. The hydrolysates of beluga visceral protein have a relatively high protein (66.43%) and low lipid (1.34%) content. The chemical score of the hydrolysate indicated that the amino acid profile of beluga sturgeon hydrolysate fulfills human adult nutritional requirements, with phenylalanine being the first limiting and predominant amino acid in the hydrolysate.