Dietary protein and carbohydrate requirement of juvenile Hawaiian limpet (Cellana sandwicensis Pease, 1861) fed practical diet


1 College of Aquaculture and Fisheries, Cantho University, Can Tho, Viet Nam

2 Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, USA


This study was conducted to evaluate dietary protein and carbohydrate requirement of juvenile Hawaiian limpets Cellana sandwincensis. A total of 64 juvenile limpets (3.12 ± 0.86 g) were fed five different dietary protein levels ranging from 270 to 470 g kg−1 for 90 days. Carbohydrate and lipid levels were held constant at 180 and 49.7 g kg−1, respectively. Weight gain and growth rates of the animals did not differ significantly (P > 0.05) among the protein levels ranging from 270 g kg−1 (0.30 % day−1) to 470 g kg−1 (0.23 % day−1). Next, opihi were fed four diets with protein levels from 210 to 500 g kg−1 with a constant carbohydrate level at 120 g kg−1. Weight gain and specific growth rates of opihi increased with increasing dietary protein from 210 to 350 g kg−1, and significantly (P < 0.05) decreased at the 500 g kg−1 diet. Highest weight gain, growth rates, and protein efficiency ratio were achieved at 350 g kg−1. Elevated carbohydrate levels (180–370 g kg−1) produced a significant difference (P < 0.05) in growth. The fastest growth rates of animals were obtained with 270 g kg−1 (0.27 % day−1) and 320 g kg−1 (0.26 % day−1). The weight gain of animals fed 180 and 370 g kg−1 carbohydrate diets were significantly (P < 0.05) lower than those of animals fed 270 and 320 g kg−1. We conclude that about 350 g kg−1 protein and 320 g kg−1 carbohydrate levels could be used for opihi.