1
National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanagawa, 236-8648, Japan
2
National Research Institute of Far Seas Fisheries, 5-7-1 Orido, Shizuoka, 424-8633, Japan
Abstract
Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed. These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was investigated using distant fish species.
Chow, S., Suzuki, N., Nakamura, Y., Yasuike, M., Saitoh, K., Yanagimoto, T. (2017). Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844). International Aquatic Research, 9(1), 53-59.
MLA
Seinen Chow; Nobuaki Suzuki; Yoji Nakamura; Motoshige Yasuike; Kenji Saitoh; Takashi Yanagimoto. "Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)". International Aquatic Research, 9, 1, 2017, 53-59.
HARVARD
Chow, S., Suzuki, N., Nakamura, Y., Yasuike, M., Saitoh, K., Yanagimoto, T. (2017). 'Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)', International Aquatic Research, 9(1), pp. 53-59.
VANCOUVER
Chow, S., Suzuki, N., Nakamura, Y., Yasuike, M., Saitoh, K., Yanagimoto, T. Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844). International Aquatic Research, 2017; 9(1): 53-59.
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