Evaluation of dietary soybean meal as fish meal replacer for juvenile whiteleg shrimp, Litopenaeus vannamei reared in biofloc system

Document Type : Original Article


1 Department of Life, Food and Ingredients, CJ Cheiljedang Center, 330 Dongho-ro, Jung-gu, Seoul, 04560, Republic of Korea

2 Department of Marine Bio-materials and Aquaculture/Feeds and Foods Nutrition Research Center, Pukyong National University, Busan, 608-737, Republic of Korea



Different levels of dietary soybean meal (SBM) as a fish meal (FM) replacer, with and without amino acid supplementation, for whiteleg shrimp, Litopenaeus vannamei reared in the biofloc system was examined in eight weeks of feeding trial. Eight experimental diets consisted of a basal diet with 0% FM replacement by SBM provided in clear sea water without biofloc system (S0SW), four diets replacing FM at 0% (S0), 33% (S33), 67% (S67) and 100% (S100) by SBM, and three diets replacing FM at 33% (S33A), 67% (S67A) and 100% (S100A) by SBM supplemented with amino acids (methionine and lysine) in the seawater biofloc system. Results of water quality analyses showed significantly lower total suspended solids and nitrate for S0SW group than all other treatments. Diets S0 and S33A resulted in higher weight gain and specific growth rate among all groups, with no significant differences with S33 group. In addition, whole-body protein and amino acid compositions of shrimp fed S0SW were lower than most biofloc groups. Haemolymph parameters showed significant differences in total protein, cholesterol and triglyceride between groups S0 and S0SW. Also, superoxide dismutase activity showed a decreasing trend with increasing replacement level. In conclusion, based on these results, SBM could replace up to 33% of FM with or without amino acid supplementation in juvenile whiteleg shrimp diets reared in the biofloc system.